Jennifer Pluznick Renal physiology; the role of sensory receptors in regulating renal function; identifying renal olfactory receptor ligands and relating them to whole-animal physiology. We are interested in the role that chemosensation plays in regulating physiological processes, particularly in the kidney. In order to maintain homeostasis, the kidney must carefully monitor and regulate the excretion of many substances – in fact, the kidney filters the entire blood volume ~30 times per day in order to help accomplish this. As an important regulator of the final plasma (and urine) concentration of many ions and other substances, the kidney would be a logical place to place an in-line chemosensor. We have found that olfactory receptors (chemoreceptors expressed in the nose, which allow you to smell) as well as other sensory receptors are expressed in the kidney. We are currently working to identify the full complement of sensory receptors in the kidney, to localize these receptors within the kidney, and to identify their ligands.
Ultimately, our goal is to understand the physiological roles that these receptors play in renal function. To accomplish this, we employ cellular and molecular biology techniques to localize the receptors and identify their ligands, as well in vivo techniques in order to investigate physiological parameters in a variety of knockout mouse models.
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Pluznick JL, Protzko RJ, Gevorgyan H, Peterlin Z, Sipos A, Han J, Brunet I, Wan LX, Rey F, Wang T, Firestein SJ, Yanagisawa M, Gordon JI, Eichmann A, Peti-Peterdi J, Caplan MJ. (2013) Olfactory receptor responding to gut microbiota-derived signals plays a role in renin secretion and blood pressure regulation. Proc Natl Acad Sci U S A. 2013 Feb 11. [Epub ahead of print]
PubMed
Pluznick JL, Caplan MJ. (2012) Novel sensory signaling systems in the kidney. Curr Opin Nephrol Hypertens. 2012 Jul;21(4):404-9. doi: 10.1097/MNH.0b013e328354a6bd. Review.
PubMed
Pluznick JL, Rodriguez-Gil DJ, Hull M, Mistry K, Gattone V, Johnson CA, Weatherbee S, Greer CA, Caplan MJ. (2011) Renal cystic disease proteins play critical roles in the organization of the olfactory epithelium. PLoS One. 2011;6(5):e19694. doi: 10.1371/journal.pone.0019694. Epub 2011 May 13. PubMed
Pluznick JL, Zou DJ, Zhang X, Yan Q, Rodriguez-Gil DJ, Eisner C, Wells E, Greer CA, Wang T, Firestein S, Schnermann J, Caplan MJ. Functional Expression of the Olfactory Signaling System in the Kidney. Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):2059-64. Epub 2009 Jan 27. PubMedLal M, Song X, Pluznick JL, Di Giovanni V, Merrick DM, Rosenblum ND, Chauvet V, Gottardi CJ, Pei Y, Caplan MJ. Polycystin-1 C-terminal tail associates with beta-catenin and inhibits canonical Wnt signaling. Hum Mol Genet. 2008 Oct 15;17(20):3105-17.
PubMed
Wang X, Pluznick JL, Settles DC, Sansom SC. Association of VASP with TRPC4 in PKG-mediated inhibition of the store-operated calcium response in mesangial cells. AJP:Renal. 2007 Dec;293(6):F1768-76.
PubMed
Pluznick JL, Sansom SC. BK channels in the kidney: role in K+ secretion and localization of molecular components. AJP:Renal. 2006 Sep; 291(3):F517-29.
PubMed
Pluznick JL, Wei P, Grimm RP, Sansom SC. The BK-β1 subunit: Immunolocalization in the mammalian connecting tubule and its role in the kaliuretic response to volume expansion. AJP:Renal. 2005 Apr; 288(4):F846-54.
PubMed
Wang X, Pluznick JL, Wei P, Padanilam BJ, Sansom SC. TRPC4 forms store-operated Ca2+ channels in mouse mesangial cells. AJP: Cell. 2004 Aug; 287(2):C357-64.
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